24324C

Sigma

 

EX-CELL® 302 Serum-Free Medium for CHO Cells

dry powder, without L-glutamine, without sodium bicarbonate

Synonym:EX-CELL® 302 Serum-Free Medium for CHO Cells

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Description

ApplicationEX-CELL® 302 is a serum-free medium which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or the GS System™, because it does not contain hypoxanthine, thymidine or L-glutamine. For use with the DHFR- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kDa. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic™ F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.
CautionDo not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.
Legal InformationEX-CELL is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

descriptionDrug Master File available
 for research or for further manufacturing use
sterilitysterile-filtered
formdry powder
storage conditionprotect from light
pH7.1-7.7
suitabilitysuitable for cell culture
storage temp.2-8°C